Question 1

A (Coulter counter/cytometer/spectrophotometer) can measure changes in the turbidity of a bacterial culture.

Accepted Answer

The answer of A (Coulter counter/cytometer/spectrophotometer) can measure changes in...

Question 2

Metabolic activity is at maximum level in the phase of growth.

Accepted Answer

A)   death
B)   intermediate
C)   lag
D)   stationary
E)   logF) A) and B)
G) None of the above E

Question 3

A(n) (culture/specimen/inoculum) is a sample of microorganisms introduced into a growth medium.

Accepted Answer

The answer of A(n) (culture/specimen/inoculum) is a sample of microorganisms...

Question 4

Alkalinophiles can survive in water up to pH (11.5/10/7.0).

Accepted Answer

The answer of Alkalinophiles can survive in water up to...

Question 5

A laboratory protocol lists the following ingredients: 1 g sucrose, 16.4 g Na 2HPO4, 1.5 g (NH4) 3PO4, 0.02 g CaCO3, KNO3, water to 1 liter and autoclave. This recipe is for a medium.

Accepted Answer

A)   complex broth
B)   defined agar
C)   defined broth
D)   complex enrichment broth
E)   reducing agarF) B) and E)
G) A) and D)

Question 6

The best method for preserving bacterial cells in storage for decades is

Accepted Answer

A)   lyophilization.
B)   deep freezing.
C)   refrigeration.
D)   drying under anaerobic conditions.
E)   storing them in shrink-wrap.F) A) and C)
G) A) and B)

Question 7

A Petroff-Hauser counting chamber is a(n)

Accepted Answer

A)   glass slide containing an etched grid for counting microbes directly using a microscope.
B)   apparatus that traps bacterial cells on a membrane filter where they can be counted.
C)   device that measures the amount of light that passes through a culture.
D)   device used to count numbers of bacterial colonies on a Petri plate.
E)   device that counts cells as they interrupt an electrical current.F) B) and C)
G) A) and D)

Question 8

The preferred method for long term storage of bacteria is (freezing/refrigeration/lyophilization).

Accepted Answer

The answer of The preferred method for long term storage...

Question 9

All of the following are part of the process of binary fission EXCEPT

Accepted Answer

A)   DNA replication.
B)   septum formation.
C)   budding.
D)   doubling of cell size.
E)   cytoplasmic membrane production.F) A) and D)
G) A) and C)

Question 10

The method provides an estimate of the CFUs (colony -forming units)  in a sample.

Accepted Answer

A)   direct microscopic-count
B)   streak-plate
C)   turbidity
D)   viable plate-count
E)   Coulter counterF) C) and D)
G) B) and E)

Question 11

Which of the following oxidizing agents is sometimes produced during aerobic respiration?

Accepted Answer

A)   peroxide anion
B)   superoxide radical
C)   singlet oxygen
D)   molecular oxygen
E)   hydroxyl radicalF) All of the above
G) A) and B)

Question 12

The are an important source of nitrogen compounds that can be metabolized by most other organisms.

Accepted Answer

A)   algae
B)   higher plants
C)   Helicobacter pylori
D)   cyanobacteria
E)   LactobacillusF) A) and B)
G) None of the above

Question 13

A growth curve plots the (number/concentration) of viable organisms in a growing population over time.

Accepted Answer

The answer of A growth curve plots the (number/concentration) of...

Question 14

Explain the similarities and differences between direct and indirect methods of measuring microbial growth, and give examples of each.

Accepted Answer

Direct methods of measuring microbial growth involve quantifying the number of microbes in a specimen through actual counting of cells in a microscope or cytometer, or counting the numbers of colonies produced from the plating of a specimen. The advantage of these techniques lies in their usefulness for counting very large and very small populations of bacteria. Indirect methods, by contrast, seek to quantify cells by measuring some characteristic related to the number of cells present. For example, spectrophotometry measures the amount of light transmitted through a culture; the less light that is transmitted, the more cells are present. The amount of light transmitted gives an approximation of the number of cells present. Indirect methods are useful for quantifying microbes, such as filamentous microbes, that are hard to count directly. Both direct and indirect methods seek to arrive at an approximation of the actual number of cells present. Because that number is changing even during the measurement process, neither technique can give an exact number of cells.

Question 15

Nitrogen fixation produces ammonia from amino acids.

Accepted Answer

A) True 
 B)False

Question 16

Creating conditions in the laboratory that promote the growth of some microbes while inhibiting the growth of others is called

Accepted Answer

A)   transport conditions.
B)   differential culturing.
C)   complex culturing.
D)   reducing conditions.
E)   selective enrichment culturing.F) C) and E)
G) B) and D) E

Question 17

The only effective way to store bacterial cultures for short periods of time is to arrest their metabolism by freezing.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following is an approach to preventing the formation of biofilms?

Accepted Answer

A)   blocking the activity of carbohydrate catabolism
B)   increasing quorum sensing
C)   triggering the production of streamers
D)   digesting matrix molecules
E)   stimulating quorum sensing receptorsF) C) and D)
G) A) and B)

Question 19

Sodium thioglycollate is found in which of the following types of media?

Accepted Answer

A)   reducing media
B)   transport media
C)   differential media
D)   selective media
E)   complex mediaF) A) and C)
G) A) and B)

Question 20

Microbial growth rates are controlled in a chemostat by

Accepted Answer

A)   maintaining a constant temperature.
B)   allowing waste products to accumulate.
C)   controlling the amount of a limiting nutrient.
D)   controlling the amount of available oxygen.
E)   promoting biofilm formation.F) C) and E)
G) B) and D)

Exam 6: Microbial Nutrition and Growth

A (Coulter counter/cytometer/spectrophotometer) can measure changes in the turbidity of a bacterial culture.

Correct Answer
verified

Metabolic activity is at maximum level in the phase of growth.

Correct Answer
verified
E

A(n) (culture/specimen/inoculum) is a sample of microorganisms introduced into a growth medium.

Correct Answer
verified

Alkalinophiles can survive in water up to pH (11.5/10/7.0).

Correct Answer
verified

A laboratory protocol lists the following ingredients: 1 g sucrose, 16.4 g Na 2HPO4, 1.5 g (NH4) 3PO4, 0.02 g CaCO3, KNO3, water to 1 liter and autoclave. This recipe is for a medium.

Correct Answer
verified

The best method for preserving bacterial cells in storage for decades is

Correct Answer
verified

A Petroff-Hauser counting chamber is a(n)

Correct Answer
verified

The preferred method for long term storage of bacteria is (freezing/refrigeration/lyophilization).

Correct Answer
verified

All of the following are part of the process of binary fission EXCEPT

Correct Answer
verified

The method provides an estimate of the CFUs (colony -forming units) in a sample.

Correct Answer
verified

Which of the following oxidizing agents is sometimes produced during aerobic respiration?

Correct Answer
verified

The are an important source of nitrogen compounds that can be metabolized by most other organisms.

Correct Answer
verified

A growth curve plots the (number/concentration) of viable organisms in a growing population over time.

Correct Answer
verified

Explain the similarities and differences between direct and indirect methods of measuring microbial growth, and give examples of each.

Nitrogen fixation produces ammonia from amino acids.

Correct Answer
verified

Creating conditions in the laboratory that promote the growth of some microbes while inhibiting the growth of others is called

Correct Answer
verified
E

The only effective way to store bacterial cultures for short periods of time is to arrest their metabolism by freezing.

Correct Answer
verified

Which of the following is an approach to preventing the formation of biofilms?

Correct Answer
verified

Sodium thioglycollate is found in which of the following types of media?

Correct Answer
verified

Microbial growth rates are controlled in a chemostat by

Correct Answer
verified

Exam 6: Microbial Nutrition and Growth

A (Coulter counter/cytometer/spectrophotometer) can measure changes in the turbidity of a bacterial culture.

Correct AnswerverifiedShow Answer

Metabolic activity is at maximum level in the phase of growth.

Correct AnswerverifiedE

A(n) (culture/specimen/inoculum) is a sample of microorganisms introduced into a growth medium.

Correct AnswerverifiedShow Answer

Alkalinophiles can survive in water up to pH (11.5/10/7.0).

Correct AnswerverifiedShow Answer

A laboratory protocol lists the following ingredients: 1 g sucrose, 16.4 g Na 2HPO4, 1.5 g (NH4) 3PO4, 0.02 g CaCO3, KNO3, water to 1 liter and autoclave. This recipe is for a medium.

Correct AnswerverifiedShow Answer

The best method for preserving bacterial cells in storage for decades is

Correct AnswerverifiedShow Answer

A Petroff-Hauser counting chamber is a(n)

Correct AnswerverifiedShow Answer

The preferred method for long term storage of bacteria is (freezing/refrigeration/lyophilization).

Correct AnswerverifiedShow Answer

All of the following are part of the process of binary fission EXCEPT

Correct AnswerverifiedShow Answer

The method provides an estimate of the CFUs (colony -forming units) in a sample.

Correct AnswerverifiedShow Answer

Which of the following oxidizing agents is sometimes produced during aerobic respiration?

Correct AnswerverifiedShow Answer

The are an important source of nitrogen compounds that can be metabolized by most other organisms.

Correct AnswerverifiedShow Answer

A growth curve plots the (number/concentration) of viable organisms in a growing population over time.

Correct AnswerverifiedShow Answer

Explain the similarities and differences between direct and indirect methods of measuring microbial growth, and give examples of each.

Nitrogen fixation produces ammonia from amino acids.

Correct AnswerverifiedShow Answer

Creating conditions in the laboratory that promote the growth of some microbes while inhibiting the growth of others is called

Correct AnswerverifiedE

The only effective way to store bacterial cultures for short periods of time is to arrest their metabolism by freezing.

Correct AnswerverifiedShow Answer

Which of the following is an approach to preventing the formation of biofilms?

Correct AnswerverifiedShow Answer

Sodium thioglycollate is found in which of the following types of media?

Correct AnswerverifiedShow Answer

Microbial growth rates are controlled in a chemostat by

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
E

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
E

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified