Question 1

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Accepted Answer

A) True 
 B)False

Question 2

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following statements concerning DNA libraries is FALSE?

Accepted Answer

A)   A genomic library is obtained by cutting the entire genome of an organism.
B)   A cDNA library is obtained by using reverse transcriptase.
C)   A DNA library contains cloned fragments of most of an organism's genome.
D)   A cDNA library lacks introns.
E)   A genomic library lacks exons.F) B) and D)
G) D) and E)

Question 4

Traditional breeding programs:

Accepted Answer

A)   involve inserting a plasmid into a host cell.
B)   involve infecting a host plant with a virus.
C)   allow gene transfers between species incapable of hybridizing.
D)   depend on inherent genetic variability.
E)   allow the precise insertion of genes into a plant.F) B) and D)
G) B) and C)

Question 5

One important characteristic the Taq polymerase is its

Accepted Answer

A)   insensitivity to pH.
B)   ability to glow when in the presence of a suitable substrate.
C)   ability to be cloned.
D)   resistance to high temperature.
E)   ability to transform cells.F) A) and B)
G) A) and C)

Question 6

Agrobacterium tumefaciens is used to obtain transgenetic plants.

Accepted Answer

A) True 
 B)False

Question 7

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following statements about eukaryotic genomes is FALSE?

Accepted Answer

A)   They are larger than those of prokaryotes.
B)   In general, multicellular eukaryotes have more DNA than unicellular eukaryotes.
C)   The greater the complexity of the organism, the more protein-coding genes it has.
D)   The roundworm Caenorhacditis has more genes than the fruit fly Drosophila.
E)   The plant Arabidopsis has more genes than the fruit fly Drosophila.F) C) and D)
G) A) and D)

Question 9

Cells containing the gene ampR would not survive when incubated on a medium containing ampicillin.

Accepted Answer

A) True 
 B)False

Question 10

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Accepted Answer

A) True 
 B)False

Question 11

Sticky-ended fragments can be joined by specific restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 12

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Accepted Answer

A) True 
 B)False

Question 13

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Accepted Answer

A) True 
 B)False

Question 14

A genetic map is different from a physical map in that a genetic map is:

Accepted Answer

A)   used to determine the function of genes.
B)   based on rates of recombination.
C)   used to determine which genes are inactivate.
D)   based on direct DNA sequencing.
E)   based on evolutionary relationships.F) All of the above
G) A) and E)

Question 15

One advantage of meristem culture is that virus-free plants can be obtained.

Accepted Answer

A) True 
 B)False

Question 16

By definition, hydroponics is the technique for growing:

Accepted Answer

A)   shoot tips.
B)   isolated leaf pieces.
C)   isolated roots
D)   plant embryos.
E)   plants without soil.F) A) and B)
G) C) and D)

Question 17

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

Accepted Answer

A)   R
B)   O
C)   onc
D)   vir
E)   AF) B) and C)
G) C) and D)

Question 18

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

Accepted Answer

A) True 
 B)False

Question 19

It was not until the discovery of ________ that organ and tissue culture became feasible.

Accepted Answer

A)   hydroponics
B)   plant hormones
C)   the importance of sucrose in nutrient solutions
D)   the essential mineral elements
E)   biotechnologyF) C) and E)
G) None of the above

Question 20

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

Accepted Answer

A)   to delay leaf senescence
B)   to confer resistance to herbicides
C)   to confer resistance to BT toxin
D)   to increase starch content
E)   to increase glyphosate productionF) B) and E)
G) None of the above

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

A genetic map is different from a physical map in that a genetic map is:

Correct AnswerverifiedShow Answer

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Correct AnswerverifiedShow Answer

Sticky-ended fragments can be joined by specific restriction enzymes.

Correct AnswerverifiedShow Answer

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Correct AnswerverifiedShow Answer

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

Correct AnswerverifiedShow Answer

By definition, hydroponics is the technique for growing:

Correct AnswerverifiedShow Answer

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Correct AnswerverifiedShow Answer

Traditional breeding programs:

Correct AnswerverifiedShow Answer

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

Correct AnswerverifiedShow Answer

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Correct AnswerverifiedShow Answer

Which of the following statements concerning DNA libraries is FALSE?

Correct AnswerverifiedShow Answer

It was not until the discovery of ________ that organ and tissue culture became feasible.

Correct AnswerverifiedShow Answer

Which of the following statements about eukaryotic genomes is FALSE?

Correct AnswerverifiedShow Answer

One advantage of meristem culture is that virus-free plants can be obtained.

Correct AnswerverifiedShow Answer

Cells containing the gene ampR would not survive when incubated on a medium containing ampicillin.

Correct AnswerverifiedShow Answer

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

Correct AnswerverifiedShow Answer

Agrobacterium tumefaciens is used to obtain transgenetic plants.

Correct AnswerverifiedShow Answer

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Correct AnswerverifiedShow Answer

One important characteristic the Taq polymerase is its

Correct AnswerverifiedShow Answer

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Correct AnswerverifiedShow Answer

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Cells containing the gene amp^R would not survive when incubated on a medium containing ampicillin.

Correct Answer
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